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KMID : 0545119940040040322
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 4 p.322 ~ p.326
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Characterization of Bacillus thuringiensis StrainBT-14 having Insecticidal Activity against Plutella xylostella
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Jung, Yong Chul
Kim, Sung Uk/Son, Kwang Hee/Lee, Hyung Hoan/Bok, Song Hae
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Abstract
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Bacillus thuringiensis strain BT-14 was isolated from alfalfa dust in Korea. The strain BT-14 produced one bipyramidal crystal and one spore in the cell. The biochemical characteristics of the strain BT-14 were similar to those of Bacillus thuringiensis subsp. kurstaki HD-1. Examination of its antibiotic resistance revealed that while the strain BT-14 was less resistant than BTK HD-1 to ampicillin, gentamycin, neomycin and tobramycin, it was more resistant to amikacin than BTK HD-1. The δ-endotoxin crystal of strain BT-14 consisted of a single protein with a high molecular weight of ca 135 KD on a 10% SDS-PACE. The strain BT-14 contained at least nine different plasmids with sizes of 2.9, 5.3, 5.8, 6.2, 9.4, 15.1, 18.1, 23.1 and 79 Kb. In insect bioassay, the isolated strain BT-14 showed lethality of 67% against Plutella xylostella larvae at dilution of 5×10 exp (-4) (5×10 to 3×10 exp (2) spores/㎖), which is almost equivalent to that of BTK HD-1.
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